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nmdcat.online June 27, 2026

An increase in the concentration of the substrate, while keeping a fixed amount of a non-competitive inhibitor, will cause the maximum reaction velocity (Vmax) to

A. Increase to the level of the uninhibited reaction
B. Remain decreased compared to the uninhibited reaction
✓
C. Decrease further than the initial inhibited rate
D. Fluctuate unpredictably with substrate concentration

📝 Explanation

A non-competitive inhibitor reduces the total amount of functional enzyme, thereby lowering the Vmax. Since the inhibitor does not bind to the active site, increasing the substrate concentration cannot saturate the inhibitor and restore Vmax to its original level.

📖 Additional Information

  • Increase to the level of the uninhibited reaction
  • Remain decreased compared to the uninhibited reaction
  • Decrease further than the initial inhibited rate
  • Fluctuate unpredictably with substrate concentration

A non-competitive inhibitor reduces the total amount of functional enzyme, thereby lowering the Vmax. Since the inhibitor does not bind to the active site, increasing the substrate concentration cannot saturate the inhibitor and restore Vmax to its original level.

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