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Written from the 3' to 5' direction (or 5'-CCA-3' read towards the 3' terminus), this terminal sequence attaches to the specific amino acid.

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Jul 3, 2026

22. The process of eukaryotic mRNA splicing involves the precise removal of

A. Exons and joining of introns
B. Introns and joining of exons ✓
C. Promoters and joining of enhancers
D. Caps and joining of tails

Introns are non-coding regions that must be excised, and exons are the coding sequences that are ligated together to form mature mRNA.

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Jul 3, 2026

21. The role played by ribosomal RNA (rRNA) during peptide bond formation is to act as a

A. Structural scaffold only
B. Ribozyme catalyst ✓
C. Genetic template
D. Codon recognition factor

The large ribosomal subunit contains rRNA that acts as a ribozyme (peptidyl transferase) to catalyze peptide bond formation.

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Jul 3, 2026

20. The enzyme responsible for transcribing ribosomal RNA (rRNA) in the eukaryotic nucleolus is

A. RNA Polymerase I ✓
B. RNA Polymerase II
C. RNA Polymerase III
D. DNA Polymerase III

In eukaryotes, RNA Polymerase I is specialized for transcribing the major ribosomal RNA genes within the nucleolus.

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Jul 3, 2026

19. The structural configuration described as a cloverleaf model represents the

A. Primary structure of mRNA
B. Secondary structure of tRNA ✓
C. Tertiary structure of rRNA
D. Quaternary structure of ribosomes

The cloverleaf pattern arises due to localized base pairing within a single tRNA strand, creating loops and stems.

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Jul 3, 2026

18. An analysis of a certain viral RNA reveals that it does not follow Chargaff’s rules because it

A. Contains uracil instead of thymine
B. Is single-stranded ✓
C. Contains ribose instead of deoxyribose
D. Is folded into secondary structures

Chargaff's rules apply only to double-stranded nucleic acids where base pairing forces a 1:1 ratio between complementary bases.

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Jul 3, 2026

17. The modification added to the 5′ end of eukaryotic pre-mRNA during processing is a

A. Poly-A tail
B. 7-methylguanosine cap ✓
C. CCA sequence
D. Intron loop

The 5' cap protects the transcript from exonuclease degradation and assists in ribosome binding during translation initiation.

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Jul 3, 2026

16. During the synthesis of an RNA strand, the incoming nucleotide is attached to the

A. 5' carbon of the existing strand
B. 3' hydroxyl group of the existing strand ✓
C. Nitrogenous base of the template strand
D. 2' hydroxyl group of the existing strand

RNA polymerase adds new nucleotides exclusively to the free 3'-OH group of the growing RNA polymer, moving in a 5' to 3' direction.

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Jul 3, 2026

15. The basic chemical difference between a ribonucleoside and a ribonucleotide is the presence of a

A. Nitrogenous base
B. Pentose sugar
C. Phosphate group ✓
D. Hydroxyl group

A nucleoside consists only of a sugar and a base; adding a phosphate group converts it into a nucleotide.

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Jul 3, 2026

14. The functional role of RNA in retroviruses such as HIV is to act as the

A. Structural catalyst
B. Primary genetic material ✓
C. Secondary messenger
D. Energy storage molecule

Retroviruses lack DNA in their viral particles and utilize RNA to store their complete hereditary information.

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Jul 3, 2026

13. In a cellular environment, the life span of messenger RNA (mRNA) compared to ribosomal RNA (rRNA) is

A. Significantly shorter ✓
B. Significantly longer
C. Exactly identical
D. Permanently stable

Messenger RNA is rapidly degraded by ribonucleases after translation to control the rate of protein synthesis dynamically.

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Jul 3, 2026

12. The type of RNA molecule that possesses an anticodon loop is

A. Messenger RNA
B. Ribosomal RNA
C. Transfer RNA ✓
D. Heterogeneous nuclear RNA

Transfer RNA contains an anticodon loop with three specific bases complementary to an mRNA codon.

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Jul 3, 2026

11. The fundamental building block of all types of ribonucleic acids is a

A. Ribonucleoside
B. Ribonucleoside monophosphate ✓
C. Deoxyribonucleotide
D. Amino acid

Ribonucleoside monophosphates (ribonucleotides) link together via condensation reactions to build the polymeric RNA strand.

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Jul 3, 2026

10. The nitrogenous base pair that exhibits two hydrogen bonds during transient RNA-DNA hybridization is

A. Adenine and Uracil ✓
B. Guanine and Cytosine
C. Adenine and Thymine
D. Cytosine and Uracil

Uracil pairs with adenine via two hydrogen bonds during transcription, mirroring the adenine-thymine interaction in DNA.

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Jul 3, 2026

9. The organic bases classified as purines found in RNA molecules are

A. Adenine and Uracil
B. Guanine and Cytosine
C. Adenine and Guanine ✓
D. Cytosine and Uracil

Purines are double-ringed nitrogenous bases, which include adenine and guanine in both DNA and RNA.

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Jul 3, 2026

8. The structural feature that allows transfer RNA (tRNA) to pick up and carry a specific amino acid is located at its

A. 5' phosphate end
B. 3' hydroxyl end ✓
C. Anticodon loop
D. Variable loop

The 3' end of tRNA terminates in a conserved CCA sequence, where the hydroxyl group of the terminal adenine binds to an amino acid.

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Jul 3, 2026

7. The structural conformation of a typical messenger RNA (mRNA) molecule is generally

A. Single-stranded and linear ✓
B. Double-stranded and helical
C. Single-stranded and cloverleaf
D. Circular and double-stranded

Messenger RNA is synthesized as a single-stranded linear molecule to allow ribosomes to read its codons sequentially.

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Jul 3, 2026

Ribosomal RNA is the most abundant form of RNA in the cell because ribosomes are present in vast numbers to meet translational demands.

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Jul 3, 2026

5. The type of RNA that carries genetic information from the nuclear DNA to the ribosomes for translation is

A. Transfer RNA
B. Ribosomal RNA
C. Messenger RNA ✓
D. Small nuclear RNA

Messenger RNA (mRNA) serves as a linear transcript of the genetic blueprint to guide amino acid sequencing during translation.

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Jul 3, 2026
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