BIO NMDCAT Structure of DNA
nmdcat.online July 3, 2026

90. An in vitro transcription assay utilizes a mutated RNA polymerase that cannot recognize or respond to the prokaryotic intrinsic termination signal. When this enzyme transcribes a gene containing a classic rho-independent terminator, the resulting molecular outcome will be

A. The production of an exceptionally short, truncated RNA molecule
B. The synthesis of an abnormally elongated RNA transcript due to read-through
C. The immediate conversion of the template DNA into a left-handed Z-conformation
D. The covalent attachment of the enzyme to the structural promoter

📝 Explanation

Without a functional termination response, the polymerase runs past the end of the gene, creating a long, un-terminated read-through transcript.

📖 Additional Information

  • The production of an exceptionally short, truncated RNA molecule
  • The synthesis of an abnormally elongated RNA transcript due to read-through
  • The immediate conversion of the template DNA into a left-handed Z-conformation
  • The covalent attachment of the enzyme to the structural promoter

Without a functional termination response, the polymerase runs past the end of the gene, creating a long, un-terminated read-through transcript.

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