Practice Questions

11. The vertical distance covered by one complete turn of the standard B-DNA double helix is

A. 0.34 nanometers
B. 2.0 nanometers
C. 3.4 nanometers
D. 20 nanometers

One complete helical turn spans a longitudinal length of 3.4 nm (34 A˚) and contains approximately 10 base pairs.

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10. In a double-stranded DNA molecule, the base-pairing pattern always obeys Chargaff’s rules, which state that the total amount of

A. Adenine equals cytosine
B. Purines equals pyrimidines
C. Thymine equals guanine
D. Uracil equals thymine

Chargaff’s rules dictate that [A]=[T] and [G]=[C]; therefore, the sum of purines (A+G) must equal the sum of pyrimidines (T+C).

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9. The scientific data that directly provided Watson and Crick with the structural dimensions and helical nature of DNA was derived from

A. Protein sequencing
B. X-ray diffraction images
C. Paper chromatography
D. Electron microscopy

Rosalind Franklin’s famous "Photo 51" obtained via X-ray crystallography revealed the helical configuration and dimensions of the DNA molecule.

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8. The chemical linkage that holds the sugar-phosphate backbone of a single DNA strand together is the

A. Hydrogen bond
B. Phosphodiester bond
C. Peptide bond
D. Glycosidic bond

Phosphodiester bonds covalently link the 3' carbon of one deoxyribose sugar to the 5' carbon of the adjacent sugar via a phosphate group.

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7. The total diameter of the standard B-form DNA double helix as determined by Watson and Crick is exactly

A. 1 nanometer
B. 2 nanometers
C. 3.4 nanometers
D. 0.34 nanometers

The double helix maintains a constant width of 2 nm (20 A˚) because a double-ringed purine always pairs with a single-ringed pyrimidine.

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Adenine (a purine) always pairs with thymine (a pyrimidine) using two hydrogen bonds in standard Watson-Crick base pairing.

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4. The chemical group located specifically at the 5′ terminus of a single DNA strand is a

A. Hydroxyl group
B. Phosphate group
C. Carboxyl group
D. Amino group

The 5' end of a DNA or RNA strand terminates in a phosphate group attached to the 5' carbon of the pentose sugar.

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A gene is the fundamental structural and functional unit of heredity, consisting of a nucleotide sequence that carries the code for a protein.

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Guanine pairs with cytosine via three hydrogen bonds, creating a more thermally stable bond than the adenine-thymine pair.

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1. According to the structural model proposed by Watson and Crick, the two strands of a DNA double helix run

A. Parallel to each other
B. Anti-parallel to each other
C. Perpendicular to each other
D. Radially outward from a core

One strand runs in the 5' to 3' direction while its complementary partner runs in the 3' to 5' direction.

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