Practice Questions

30. The major structural component of the small ribosomal subunit in prokaryotes is

A. 18S rRNA
B. 23S rRNA
C. 16S rRNA
D. 5S rRNA

The prokaryotic 30S small ribosomal subunit contains the 16S rRNA molecule, which plays a key role in identifying the Shine-Dalgarno sequence.

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29. The phenomenon where a single tRNA species can recognize more than one codon on mRNA is explained by the

A. Wobble hypothesis
B. Central dogma
C. Splicing mechanism
D. Degeneracy of genetic code

The wobble hypothesis states that non-standard base pairing can occur between the third base of a codon and the first base of an anticodon.

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28. The intramolecular base pairing within a transfer RNA molecule is stabilized by

A. Covalent disulfide bridges
B. Hydrophobic interactions
C. Hydrogen bonds
D. Phosphodiester linkages

Complementary bases within the single-stranded tRNA bend back and form hydrogen bonds, creating its characteristic shapes.

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AUG is the universal start codon that codes for methionine in eukaryotes and formyl-methionine in prokaryotes.

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26. In prokaryotes, translation of an mRNA molecule can begin before transcription is complete due to the

A. Absence of a nuclear membrane
B. Simpler structure of prokaryotic tRNA
C. Absence of introns in prokaryotic DNA
D. High speed of prokaryotic RNA polymerase

Without a nuclear envelope separating DNA from ribosomes, prokaryotes can simultaneously transcribe and translate a genetic message.

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25. The presence of a poly-A tail at the 3′ end of a eukaryotic mRNA molecule functions to

A. Direct the ribosome to the start codon
B. Promote splicing of the final intron
C. Enhance stability and resist cytoplasmic degradation
D. Initiate transcription termination

The poly-A tail protects mRNA from 3' exonucleases, extending its operational lifespan within the cytoplasm.

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24. An increase in the concentration of cytoplasmic aminoacyl-tRNA synthetase directly influences the

A. Transcription of tRNA genes
B. Attachment of amino acids to tRNA
C. Splicing of pre-mRNA transcripts
D. Binding of mRNA to ribosomes

Aminoacyl-tRNA synthetase is the specific enzyme that catalyzes the esterification of a specific amino acid to its cognate tRNA.

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Written from the 3' to 5' direction (or 5'-CCA-3' read towards the 3' terminus), this terminal sequence attaches to the specific amino acid.

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22. The process of eukaryotic mRNA splicing involves the precise removal of

A. Exons and joining of introns
B. Introns and joining of exons
C. Promoters and joining of enhancers
D. Caps and joining of tails

Introns are non-coding regions that must be excised, and exons are the coding sequences that are ligated together to form mature mRNA.

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21. The role played by ribosomal RNA (rRNA) during peptide bond formation is to act as a

A. Structural scaffold only
B. Ribozyme catalyst
C. Genetic template
D. Codon recognition factor

The large ribosomal subunit contains rRNA that acts as a ribozyme (peptidyl transferase) to catalyze peptide bond formation.

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