Practice Questions

20. The enzyme responsible for transcribing ribosomal RNA (rRNA) in the eukaryotic nucleolus is

A. RNA Polymerase I
B. RNA Polymerase II
C. RNA Polymerase III
D. DNA Polymerase III

In eukaryotes, RNA Polymerase I is specialized for transcribing the major ribosomal RNA genes within the nucleolus.

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19. The structural configuration described as a cloverleaf model represents the

A. Primary structure of mRNA
B. Secondary structure of tRNA
C. Tertiary structure of rRNA
D. Quaternary structure of ribosomes

The cloverleaf pattern arises due to localized base pairing within a single tRNA strand, creating loops and stems.

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18. An analysis of a certain viral RNA reveals that it does not follow Chargaff’s rules because it

A. Contains uracil instead of thymine
B. Is single-stranded
C. Contains ribose instead of deoxyribose
D. Is folded into secondary structures

Chargaff's rules apply only to double-stranded nucleic acids where base pairing forces a 1:1 ratio between complementary bases.

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17. The modification added to the 5′ end of eukaryotic pre-mRNA during processing is a

A. Poly-A tail
B. 7-methylguanosine cap
C. CCA sequence
D. Intron loop

The 5' cap protects the transcript from exonuclease degradation and assists in ribosome binding during translation initiation.

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16. During the synthesis of an RNA strand, the incoming nucleotide is attached to the

A. 5' carbon of the existing strand
B. 3' hydroxyl group of the existing strand
C. Nitrogenous base of the template strand
D. 2' hydroxyl group of the existing strand

RNA polymerase adds new nucleotides exclusively to the free 3'-OH group of the growing RNA polymer, moving in a 5' to 3' direction.

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15. The basic chemical difference between a ribonucleoside and a ribonucleotide is the presence of a

A. Nitrogenous base
B. Pentose sugar
C. Phosphate group
D. Hydroxyl group

A nucleoside consists only of a sugar and a base; adding a phosphate group converts it into a nucleotide.

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14. The functional role of RNA in retroviruses such as HIV is to act as the

A. Structural catalyst
B. Primary genetic material
C. Secondary messenger
D. Energy storage molecule

Retroviruses lack DNA in their viral particles and utilize RNA to store their complete hereditary information.

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13. In a cellular environment, the life span of messenger RNA (mRNA) compared to ribosomal RNA (rRNA) is

A. Significantly shorter
B. Significantly longer
C. Exactly identical
D. Permanently stable

Messenger RNA is rapidly degraded by ribonucleases after translation to control the rate of protein synthesis dynamically.

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12. The type of RNA molecule that possesses an anticodon loop is

A. Messenger RNA
B. Ribosomal RNA
C. Transfer RNA
D. Heterogeneous nuclear RNA

Transfer RNA contains an anticodon loop with three specific bases complementary to an mRNA codon.

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11. The fundamental building block of all types of ribonucleic acids is a

A. Ribonucleoside
B. Ribonucleoside monophosphate
C. Deoxyribonucleotide
D. Amino acid

Ribonucleoside monophosphates (ribonucleotides) link together via condensation reactions to build the polymeric RNA strand.

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