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BIO NMDCAT Ribonucleic acid (RNA)
nmdcat.online July 3, 2026

100. An in vitro transcription reaction is carried out using a modified nucleotide mixture where the 2′ hydroxyl group of all ribose sugars is replaced by a fluorine atom (2′-F). The resulting polynucleotide product will exhibit

A. An absolute inability to form Watson-Crick base pairs with DNA
B. Complete resistance to ribonuclease-mediated alkaline hydrolysis
✓
C. Spontaneous conversion into a triple-stranded helical conformation
D. A total dependency on Rho protein factors for transcription initiation

📝 Explanation

Replacing the 2'-OH group removes the nucleophile required for autophilic attack, making the synthetic RNA highly resistant to ribonuclease degradation.

📖 Additional Information

  • An absolute inability to form Watson-Crick base pairs with DNA
  • Complete resistance to ribonuclease-mediated alkaline hydrolysis
  • Spontaneous conversion into a triple-stranded helical conformation
  • A total dependency on Rho protein factors for transcription initiation

Replacing the 2'-OH group removes the nucleophile required for autophilic attack, making the synthetic RNA highly resistant to ribonuclease degradation.

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