BIO NMDCAT Factors that Affect the Rate of Enzyme Reactions
nmdcat.online July 11, 2026

A temperature increase beyond the optimum value causes enzyme activity to decline primarily because

A. Substrate molecules become inactive
B. The active site loses its specific three-dimensional shape
C. More enzyme-substrate complexes are formed
D. The activation energy becomes zero

📝 Explanation

High temperature disrupts hydrogen bonds and other weak interactions responsible for maintaining enzyme structure, causing denaturation and loss of catalytic function.

📖 Additional Information

  • Substrate molecules become inactive
  • The active site loses its specific three-dimensional shape
  • More enzyme-substrate complexes are formed
  • The activation energy becomes zero

High temperature disrupts hydrogen bonds and other weak interactions responsible for maintaining enzyme structure, causing denaturation and loss of catalytic function.

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