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BIO NMDCAT Factors that Affect the Rate of Enzyme Reactions
nmdcat.online July 11, 2026

During enzyme action, the limiting factor changes from substrate concentration to enzyme concentration because

A. Nearly all active sites become occupied by substrate molecules
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B. Product concentration becomes zero
C. Temperature decreases continuously
D. The enzyme changes its molecular weight

📝 Explanation

Initially, substrate concentration limits the reaction. After saturation, enzyme concentration becomes the limiting factor.

📖 Additional Information

  • Nearly all active sites become occupied by substrate molecules
  • Product concentration becomes zero
  • Temperature decreases continuously
  • The enzyme changes its molecular weight

Initially, substrate concentration limits the reaction. After saturation, enzyme concentration becomes the limiting factor.

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