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BIO NMDCAT Factors that Affect the Rate of Enzyme Reactions
nmdcat.online July 11, 2026

During enzyme assays, substrate concentration is considered the limiting factor whenever

A. Active sites remain unoccupied because substrate molecules are insufficient
✓
B. All active sites are permanently occupied
C. Enzyme molecules are denatured
D. Product concentration becomes zero

📝 Explanation

When substrate is limiting, many enzyme molecules remain free, preventing the reaction from reaching its maximum rate.

📖 Additional Information

  • Active sites remain unoccupied because substrate molecules are insufficient
  • All active sites are permanently occupied
  • Enzyme molecules are denatured
  • Product concentration becomes zero

When substrate is limiting, many enzyme molecules remain free, preventing the reaction from reaching its maximum rate.

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