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nmdcat.online July 11, 2026

In a coupled assay system, enzyme X generates a product that is the substrate for enzyme Y. The activity of enzyme X is measured by the rate of product formation by enzyme Y, which is a direct measure of

A. The Km of enzyme Y for its substrate
B. The activity of enzyme X
✓
C. The Vmax of enzyme Y in isolation
D. The affinity of enzyme X for a cofactor

📝 Explanation

In a coupled assay where enzyme Y and its substrates are in excess, the rate of product formation by Y is proportional to the rate at which X provides its substrate.

📖 Additional Information

  • The Km of enzyme Y for its substrate
  • The activity of enzyme X
  • The Vmax of enzyme Y in isolation
  • The affinity of enzyme X for a cofactor

In a coupled assay where enzyme Y and its substrates are in excess, the rate of product formation by Y is proportional to the rate at which X provides its substrate.

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