BIO NMDCAT Carbohydrates
nmdcat.online June 27, 2026

The Benedict’s test is routinely used for the qualitative detection of glucose in urine for diabetes diagnosis. The principle of this test relies on the ability of glucose to act as a reducing agent in a

A. Strongly acidic solution
B. Neutral aqueous solution
C. Hot alkaline solution
D. Cold chloroform solution

📝 Explanation

Benedict's reagent is an alkaline solution of copper(II) sulfate and sodium carbonate. In a hot alkaline environment, glucose's carbonyl group reduces Cu²⁺ to Cu⁺, forming a colored precipitate. The alkalinity is crucial for the reaction to proceed.

📖 Additional Information

  • Strongly acidic solution
  • Neutral aqueous solution
  • Hot alkaline solution
  • Cold chloroform solution

Benedict's reagent is an alkaline solution of copper(II) sulfate and sodium carbonate. In a hot alkaline environment, glucose's carbonyl group reduces Cu²⁺ to Cu⁺, forming a colored precipitate. The alkalinity is crucial for the reaction to proceed.

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