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nmdcat.online July 11, 2026

The initial rate of an enzymatic reaction is measured. Doubling the enzyme concentration is found to double the initial rate. This observation is valid only when

A. The substrate is in limiting concentration
B. The enzyme is saturated with the substrate
C. The substrate is present in large excess over the enzyme
✓
D. The reaction is near equilibrium

📝 Explanation

When substrate is in excess, the reaction rate is directly proportional to enzyme concentration because every additional enzyme molecule can contribute to the product formation rate.

📖 Additional Information

  • The substrate is in limiting concentration
  • The enzyme is saturated with the substrate
  • The substrate is present in large excess over the enzyme
  • The reaction is near equilibrium

When substrate is in excess, the reaction rate is directly proportional to enzyme concentration because every additional enzyme molecule can contribute to the product formation rate.

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