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nmdcat.online June 27, 2026

The molecule agarose, a purified component of agar, is a linear polymer of alternating D-galactose and 3,6-anhydro-L-galactose. Its use in gel electrophoresis is based on its ability to form a porous gel matrix that separates macromolecules based on their

A. Optical activity
B. Size and shape
✓
C. Reducing power
D. Lipid solubility

📝 Explanation

The gel matrix of agarose acts as a molecular sieve. During electrophoresis, charged macromolecules (like DNA) move through the pores. Smaller molecules move faster and farther, while larger molecules are retarded. This sieving separates the molecules by size.

📖 Additional Information

  • Optical activity
  • Size and shape
  • Reducing power
  • Lipid solubility

The gel matrix of agarose acts as a molecular sieve. During electrophoresis, charged macromolecules (like DNA) move through the pores. Smaller molecules move faster and farther, while larger molecules are retarded. This sieving separates the molecules by size.

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