Practice Questions

72. The presence of two hydrogen bonds between Adenine and Thymine compared to three between Guanine and Cytosine means that A-T rich regions of a gene

A. Are exceptionally rigid and resist unzipping
B. Melt and separate more easily under thermal or mechanical stress
C. Contain higher quantities of deoxyribose sugars
D. Cannot be transcribed by bacterial RNA polymerases

Having fewer hydrogen bonds makes A-T rich regions easier to unzip, which is why replication origins and promoters are rich in A-T pairs.

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B-DNA is the standard, stable conformation adopted by native DNA molecules inside the aqueous environment of living cells.

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70. The definition of a cistron, which is often used interchangeably with the structural term “gene,” is a segment of DNA that

A. Codes for a single functional transfer RNA molecule only
B. Specifies the complete nucleotide sequence of a single polypeptide chain
C. Regulates the packing of histones in chromatin
D. Initiates DNA replication at a fixed chromosomal origin

A cistron is a genetic unit defined by the cis-trans position test, matching the DNA sequence needed to build one polypeptide.

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The wider, deeper gap on the outside of the helix is the major groove, which provides easy access for sequence-specific DNA-binding proteins.

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68. The chemical component of a deoxyribonucleotide that is modified during the epigenetic process of DNA methylation is the

A. Phosphate group
B. Deoxyribose sugar ring
C. Nitrogenous base (typically Cytosine)
D. Phosphodiester backbone

DNA methyltransferases add a methyl group to the carbon-5 position of cytosine rings, a key modification for silencing gene expression.

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67. The functional consequence of a mutation that deletes the promoter region of a structural gene is that the gene will

A. Be transcribed continuously at an accelerated rate
B. Fail to be transcribed because RNA polymerase cannot bind
C. Produce a protein consisting entirely of lipids
D. Be converted into an alternative allele

Without a functional promoter sequence, RNA polymerase cannot locate or bind the gene, shutting down transcription entirely.

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66. The spatial arrangement where the nitrogenous bases are tucked inside the double helix protects them from

A. Interacting with regulatory transcription factors
B. Chemical damage and modification by reactive molecules in the cell
C. Forming hydrogen bonds during replication
D. Being recognized by complementary tRNA molecules

Enclosing the genetic bases within the hydrophobic core protects the fragile hereditary codes from reactive molecules in the cytoplasm.

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By showing that viral DNA (32P) enters the host cell while viral protein (35S) stays outside, they proved DNA is the hereditary material.

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The template strand runs anti-parallel and complementary to the coding strand, making the matching sequence 3'-TACGTT-5'.

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The spliceosome is a large ribonucleoprotein complex that removes introns and splices exons together to form mature mRNA.

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