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BIO NMDCAT CELL STRUCTURE & FUNCTION
nmdcat.online July 3, 2026

90. An in vitro biochemical assay demonstrates that the tonoplast of a plant cell contains an abundance of H+-ATPase pumps that actively transport protons from the cytosol into the vacuole. This active transport mechanism establishes a

A. Basic pH inside the vacuole relative to the cytoplasm
B. Highly acidic intra-vacuolar environment essential for activating hydrolytic enzymes
✓
C. Hydrophobic core that excludes all dissolved mineral ions
D. Torsional strain that triggers the immediate destruction of plastids

📝 Explanation

Pumping protons into the central vacuole lowers its internal pH, creating an acidic environment that powers lysosome-like digestive enzymes.

📖 Additional Information

  • Basic pH inside the vacuole relative to the cytoplasm
  • Highly acidic intra-vacuolar environment essential for activating hydrolytic enzymes
  • Hydrophobic core that excludes all dissolved mineral ions
  • Torsional strain that triggers the immediate destruction of plastids

Pumping protons into the central vacuole lowers its internal pH, creating an acidic environment that powers lysosome-like digestive enzymes.

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