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BIO NMDCAT Carbohydrates
nmdcat.online June 27, 2026

During vigorous exercise, the rapid mobilization of glucose from muscle glycogen is achieved by the coordinated action of glycogen phosphorylase and the debranching enzyme. The function of the debranching enzyme is to

A. Add glucose residues to the non-reducing ends
B. Hydrolyze the α-1,4 bonds to release free glucose
C. Transfer a short oligosaccharide chain and then hydrolyze the α-1,6 glycosidic bond at the branch point
✓
D. Phosphorylate glucose to trap it inside the cell

📝 Explanation

Glycogen phosphorylase cannot cleave near a branch point. The debranching enzyme has two activities: first, transferase activity moves a short α-1,4-linked chain to a nearby non-reducing end; second, α-1,6-glucosidase activity hydrolyzes the remaining α-1,6 bond, releasing a free glucose molecule.

📖 Additional Information

  • Add glucose residues to the non-reducing ends
  • Hydrolyze the α-1,4 bonds to release free glucose
  • Transfer a short oligosaccharide chain and then hydrolyze the α-1,6 glycosidic bond at the branch point
  • Phosphorylate glucose to trap it inside the cell

Glycogen phosphorylase cannot cleave near a branch point. The debranching enzyme has two activities: first, transferase activity moves a short α-1,4-linked chain to a nearby non-reducing end; second, α-1,6-glucosidase activity hydrolyzes the remaining α-1,6 bond, releasing a free glucose molecule.

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