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BIO NMDCAT Factors that Affect the Rate of Enzyme Reactions
nmdcat.online July 11, 2026

In an enzyme assay, doubling both enzyme concentration and substrate concentration under suitable conditions generally results in

A. Complete inhibition of the reaction
B. A substantial increase in reaction rate because both active sites and substrate molecules increase
✓
C. No change in reaction rate
D. Immediate denaturation of the enzyme

📝 Explanation

Increasing both enzyme and substrate together provides more catalytic sites and sufficient substrate, leading to a marked increase in reaction rate until another factor becomes limiting.

📖 Additional Information

  • Complete inhibition of the reaction
  • A substantial increase in reaction rate because both active sites and substrate molecules increase
  • No change in reaction rate
  • Immediate denaturation of the enzyme

Increasing both enzyme and substrate together provides more catalytic sites and sufficient substrate, leading to a marked increase in reaction rate until another factor becomes limiting.

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