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BIO NMDCAT Biological Importance of Water
nmdcat.online June 27, 2026

Regarding water as a reagent in metabolic reactions, its involvement in the Krebs cycle occurs during the

A. Decarboxylation of pyruvate to acetyl-CoA
B. Hydration of fumarate to form malate
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C. Reduction of NAD⁺ to NADH
D. Substrate-level phosphorylation of GDP

📝 Explanation

In the Krebs cycle, the enzyme fumarase catalyzes the addition of a water molecule across the double bond of fumarate. This hydration reaction converts fumarate to malate. Water is a direct reactant in this specific step, not just a solvent.

📖 Additional Information

  • Decarboxylation of pyruvate to acetyl-CoA
  • Hydration of fumarate to form malate
  • Reduction of NAD⁺ to NADH
  • Substrate-level phosphorylation of GDP

In the Krebs cycle, the enzyme fumarase catalyzes the addition of a water molecule across the double bond of fumarate. This hydration reaction converts fumarate to malate. Water is a direct reactant in this specific step, not just a solvent.

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