The formation of ice crystals and the 9% volume expansion upon freezing can physically rupture cell membranes and delicate tissue structures. This is the basis of frostbite and why cryoprotective agents are needed to preserve cells.
The formation of ice crystals and the 9% volume expansion upon freezing can physically rupture cell membranes and delicate tissue structures. This is the basis of frostbite and why cryoprotective agents are needed to preserve cells.