MCQs

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Practice Questions

93. An animal cell line is engineered to completely block the expression of the protein vinculin, which is a structural component of focal adhesions. This genetic modification will directly impair the cell’s ability to

A. Form a functional cleavage furrow during mitotic division
B. Adhere to the extracellular matrix and migrate across surfaces
C. Synthesize cholesterol molecules inside the smooth ER
D. Route lysosomal enzymes from the Golgi to the lysosome

Vinculin helps anchor internal actin microfilaments to transmembrane integrins, a structural link required for cell-matrix adhesion and motility.

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92. A plant developmental biologist studies a mutant strain of Arabidopsis thaliana that lacks functional expansin proteins. Phenotypically, this mutation results in severe dwarfism because expansins are the mandatory wall proteins that

A. Catalyze the synthesis of secondary lignin complexes
B. Loosen the hydrogen bonds between cellulose microfibrils to permit turgor-driven wall extension
C. Replicate the circular genome of internal chloroplasts
D. Anchor the desmotubule inside the plasmodesmata pores

Expansins disrupt the non-covalent links between cellulose and hemicellulose, allowing turgor pressure to expand the primary cell wall.

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Jul 3, 2026

91. A patient presenting with muscle weakness and exercise intolerance is diagnosed with a mitochondrial myopathy caused by a point mutation in a mitochondrial tRNA gene. This metabolic disease affects both plant and animal cell lines because mitochondria

A. Are entirely dependent on nuclear genes for all their proteins
B. Possess their own independent, circular DNA and semi-autonomous translation machinery
C. Do not require ATP synthesis to survive
D. Are formed via the direct fusion of lysosomal vesicles

Mitochondria contain their own circular genome and ribosomes; mutations in their tRNA disrupt internal protein translation and ATP synthesis.

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Jul 3, 2026

90. An in vitro biochemical assay demonstrates that the tonoplast of a plant cell contains an abundance of H+-ATPase pumps that actively transport protons from the cytosol into the vacuole. This active transport mechanism establishes a

A. Basic pH inside the vacuole relative to the cytoplasm
B. Highly acidic intra-vacuolar environment essential for activating hydrolytic enzymes
C. Hydrophobic core that excludes all dissolved mineral ions
D. Torsional strain that triggers the immediate destruction of plastids

Pumping protons into the central vacuole lowers its internal pH, creating an acidic environment that powers lysosome-like digestive enzymes.

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Cellulase breaks down the cellulose microfibrils, and pectinase dissolves the middle lamella, releasing wall-free protoplasts.

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88. A genetic mutation disrupts the synthesis of clathrin proteins inside an animal cell line. Biochemically, this defect will lead to the selective arrest of

A. Simple passive diffusion of carbon dioxide gases
B. Receptor-mediated endocytosis and coated-vesicle budding
C. The synthesis of ribosomal subunits inside the nucleolus
D. The transport of electrons within the cristae

Clathrin is required to structuralize and coat invaginating pits during endocytosis; its absence blocks the internalization of specific ligands.

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Jul 3, 2026

87. A researcher treats a culture of dividing animal cells with the drug cytochalasin B, which selectively inhibits the polymerization of actin microfilaments. The immediate operational outcome observed in these cells will be the

A. Total arrest of nuclear envelope breakdown during prophase
B. Complete failure of cytokinesis, leaving binucleated cells
C. Inhibition of chromosome movement toward the spindle poles
D. Disruption of the mitochondrial electron transport chain

Inhibiting actin prevents the assembly of the contractile ring, blocking cleavage furrow formation and leaving the cell with two nuclei.

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Jul 3, 2026

86. An experimental plant cell line contains a loss-of-function mutation in the gene encoding the enzyme cellulose synthase. During cell division and growth, this molecular defect will directly manifest as the

A. Inability of the cell to replicate its mitochondrial DNA
B. Structural failure and bursting of newly formed daughter cells during expansion
C. Failure of the spindle apparatus to segregate sister chromatids
D. Sudden transformation of chloroplasts into amyloplasts

Without functional cellulose synthase complexes, the cell cannot build a primary cell wall, causing expanding cells to burst under turgor pressure.

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85. The biochemical nature of the enzyme catalase, which resides inside the peroxisomes of both plant and animal cells, is a

A. Structural lipid matrix
B. Conjugated globular protein (enzyme)
C. Unbranched homopolysaccharide
D. Single-stranded ribozyme

Catalase is a tetrameric protein enzyme containing four heme groups, optimized to break down hydrogen peroxide into water and oxygen.

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Jul 3, 2026

84. The primary biochemical function of the carbohydrate component that forms the outer glycocalyx coating of animal cells is

A. Serving as a template for DNA replication
B. Cell-to-cell recognition and immune system identification
C. Driving the electron transport chain
D. Storing high-density triacylglycerol reserves

The branched sugar chains of the glycocalyx act as molecular tags, enabling the immune system to recognize self cells and coordinate interactions.

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Integrins are transmembrane receptor proteins that link the intracellular cytoskeleton to the extracellular matrix, facilitating cell signaling.

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82. The fundamental difference in the structural response of a plant cell versus an animal cell to a highly hypertonic external fluid environment is that

A. The plant cell bursts while the animal cell stays stable
B. The plant cell experiences plasmolysis while keeping its outer shape, whereas the animal cell shrinks completely
C. The plant cell undergoes immediate binary fission
D. The animal cell synthesizes a protective cellulose wall

The plant cell's protoplast shrinks inward while its rigid wall maintains the outer cell shape; the wall-less animal cell shrivels up completely.

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Jul 3, 2026

81. The direct cellular source of the energy currency molecule ATP required to run active transport pumps across the plasma membrane of an animal cell is

A. Photophosphorylation in chloroplasts
B. Oxidative phosphorylation in mitochondria
C. Hydrolysis of structural cellulose
D. Fusion of lysosomal vesicles

Mitochondria harvest chemical energy from food molecules via cellular respiration, producing ATP to power energy-dependent cell processes.

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80. The dynamic structural component that lines the inner surface of the nuclear envelope in animal cells to provide mechanical support and anchor chromatin is the

A. Actin cortex
B. Nuclear lamina (composed of intermediate filaments)
C. Cell wall matrix
D. Cortical microtubule ring

The nuclear lamina is an interlaced protein mesh made of lamins that supports the nuclear envelope and organizes internal chromatin.

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79. The organelle that features a continuous network of flattened membrane sheets studded with ribosomes, highly developed in both animal and plant secretory cells, is the

A. Smooth Endoplasmic Reticulum
B. Rough Endoplasmic Reticulum
C. Central Vacuole
D. Dictyosome subunit

The rough ER is studded with active ribosomes, serving as the primary site for translating and folding proteins destined for membranes or export.

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78. The structural feature known as a “pit” in mature, secondary plant cell walls represents a localized region where

A. The plasma membrane has ruptured completely
B. No secondary cell wall material was deposited, leaving only the primary wall
C. Lignin has dissolved the middle lamella
D. Large centrioles are exported from the cell

Pits are thin depressions in the secondary wall where only the primary wall and middle lamella remain, allowing water to pass between cells.

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Jul 3, 2026

77. The structural layout of the microfilaments and microtubules inside both animal and plant cells, which provides internal structural support, is the

A. Extracellular matrix
B. Cytoskeleton
C. Phragmoplast network
D. Tonoplast framework

The cytoskeleton is a dynamic network of microfilaments, intermediate filaments, and microtubules that governs cell shape and movement.

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Jul 3, 2026

76. The metabolic process that occurs within the fluid stroma of a plant cell’s chloroplast is the

A. Krebs Cycle (Citric Acid Cycle)
B. Calvin Cycle (Light-independent reactions)
C. Glycolysis pathway
D. Electron Transport Chain

The stroma contains the soluble enzymes (such as RuBisCO) required to fix carbon dioxide into sugars during the Calvin cycle.

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Jul 3, 2026

75. The mechanism by which large, polar hydrophilic molecules (like glucose or amino acids) cross the plasma membrane of both animal and plant cells along their concentration gradient is

A. Simple diffusion
B. Facilitated diffusion via transport proteins
C. Active transport utilizing ATP
D. Endocytosis

Polar molecules cannot pass through the hydrophobic lipid core directly; they require channel or carrier proteins to cross without using energy.

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